SMART
A Specific Methylation Analysis and Report Tool for BS-Seq data
Time-stamp: <2014-08-01 22:17:24 Hongbo Liu>
Please check the following instructions to complete your installation.
Python version must be equal to 2.7 to run SMART-BS-Seq. I recommend using the version 2.7.6.
scipy (>=0.13) are required to run SMART-BS-Seq.
The easiest way to install SMART-BS-Seq is through PyPI system. Get pip if it's not available in your system. Note if you have already installed numpy and scipy system-wide, you can use `virtualenv
--system-site-packages` to let your virtual Python environment have access to system-wide numpy and scipy libraries so that you don't need to install them again.
Then under command line, type `pip install SMART-BS-Seq`. PyPI will install Numpy and Scipy automatically if they are absent.
To upgrade SMART-BS-Seq, type `pip install -U SMART-BS-Seq`. It will check currently installed SMART-BS-Seq, compare the version with the one on PyPI repository, download and install newer version while necessary.
Note, if you do not want pip to fix dependencies. For example, you already have a workable Scipy and Numpy, and when 'pip install -U SMART-BS-Seq', pip downloads newest Scipy and Numpy but unable to compile and install them. This will fail the whole installation. You can pass '--no-deps' option to pip and let it skip all dependencies. Type `pip install -U --no-deps SMART-BS-Seq`.
SMART-BS-Seq uses Python's distutils tools for source installations. To install a source distribution of SMART-BS-Seq, unpack the distribution tarball and open up a command terminal. Go to the directory where you unpacked SMART-BS-Seq, and simply run the install script:
$ python setup.py install
By default, the script will install python library and executable codes globally, which means you need to be root or administrator of the machine so as to complete the installation. Please contact the administrator of that machine if you want their help. If you need to provide a nonstandard install prefix, or any other nonstandard options, you can provide many command line options to the install script. Use the ¨Chelp option to see a brief list of available options:
$ python setup.py --help
For example, if I want to install everything under my own HOME directory, use this command:
$ python setup.py install --prefix /home/hbliu/
After running the setup script, you might need to add the install location to your PYTHONPATH and PATH environment variables. The process for doing this varies on each platform, but the general concept is the same across platforms.
To set up your PYTHONPATH environment variable, you'll need to add the value PREFIX/lib/pythonX.Y/site-packages to your existing PYTHONPATH. In this value, X.Y stands for the major¨Cminor version of Python you are using (such as 2.7 ; you can find this with sys.version[:3] from a Python command line). PREFIX is the install prefix where you installed SMART-BS-Seq. If you did not specify a prefix on the command line, SMART-BS-Seq will be installed using Python's sys.prefix value.
Using Windows, you need to open up the system properties dialog, and locate the tab labeled Environment. Add your value to the PYTHONPATH variable, or create a new PYTHONPATH variable if there isn't one already.
Just like your PYTHONPATH, you'll also need to add a new value to your PATH environment variable so that you can use the SMART-BS-Seq command line directly. Unlike the PYTHONPATH value, however, this time you'll need to add PREFIX/bin to your PATH environment variable. The process for updating this is the same as described above for the PYTHONPATH variable:
$ export PATH=/home/hbliu/bin:$PATH
| For any help: | you are welcome to write to Hongbo Liu (hongbo919@gmail.com). |
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